The present research work aims to develop a simple, sensitive, accurate and reproducible method for the simultaneous estimation of Alprazolam and Omeprazole by two different spectroscopic method viz, Simultaneous equation Method (Vierodt's method) (Method-A) and Q-Absorption method (Method-B). Absorbance maxima for Alprazolam and Omeprazole were found to be at 221 nm and 301 nm respectively for method-A, and Iso-absorptive point for both drugs was found to be 261.2 nm for method-B by using Methanol as a solvent. Absorption maxima of Omeprazole at 301nm, was taken as second wavelength for determination by method-B. Linearity for both the methods was observed in concentration range of 1-5 µg/ml for both drug Alprazolam and Omeprazole. The correlation coefficients for both the methods obtained were near to 1. The accuracy of both methods was evaluated by recovery studies and recovery results were obtained from 98 % to 102% for both the methods and the relative standard deviations below 2% were achieved. Validation was done as per ICH guidelines. Proposed methods can estimate Alprazolam and Omeprazole simultaneously in combined dosage form without the interference of common excipients.
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